EXHIBIT 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in the Registration Statement on Form N-2 (No. 333-232124) of Gladstone Investment Corporation of our report dated May 12, 2020 relating to the financial statements and financial statement schedule and our report dated May 12, 2020 related to the senior securities table, which appear in this Form 10-K. We also consent to the reference to us under the heading “Senior Securities” in this Form 10-K.

 

/s/ PricewaterhouseCoopers LLP

McLean, Virginia

May 12, 2020