Exhibit 32.1
CERTIFICATION OF THE CHIEF EXECUTIVE OFFICER PURSUANT TO
SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002
The undersigned, the Chief Executive Officer of Gladstone Investment Corporation (the Company),
hereby certifies on the date hereof, pursuant to 18 U.S.C. §1350(a), as adopted pursuant to Section
906 of The Sarbanes-Oxley Act of 2002, that the Quarterly Report on Form 10-Q for the quarter ended
June 30, 2005 (Form 10-Q), filed concurrently herewith by the Company, fully complies with the
requirements of Section 13(a) or Section 15(d) of the Securities Exchange Act of 1934, as amended,
and that the information contained in the Form 10-Q fairly presents, in all material respects, the
financial condition and results of operations of the Company.
Dated: August 10, 2005
/s/ David Gladstone
David Gladstone
Chief Executive Officer